Culture conditions molasses powder: 30 ℃, speed 200r / min, liquid volume 30mL / 250mL flask, was added in one CaCO_310.0g / L, to adjust the pH value of the seed culture time 12h; cane molasses fermentation medium is: the initial sugar concentration of 80.0g / L of sugar cane molasses, NH_4NO_30.6g / L, ZnSO_4 · 7H_2O 0.008g / L, plus a bottle of bagasse 1.5g. The optimum fermentation conditions were: 30 ℃, speed 200r / min, liquid volume of 50mL / 250mL flask, CaCO_370.0g / L (plus) to adjust the pH of the fermentation broth, inoculum was 10%. Under optimum conditions molasses powder, the fermentation of the mutant strain accumulation of lactic acid can be achieved L- 69.76g / L. 4. Because sugar cane molasses ingredient complex, high impurity content, and some hazardous substances can inhibit the production of Rhizopus L- lactic acid, it must be processed sugar cane molasses, cane molasses choose Rhizopus L- lactic acid fermentation processing molasses powder method this test determines the processing of sugar cane molasses approach.
Sulfuric acid + activated carbon + law + NaHSO_3 France potassium ferrocyanide, cane molasses after treatment with this method is used to dilute fermentation, lactic acid production at its L- by untreated 52.34g / L increased to 61.88g / L, production increased by about 18%. reducing molasses powder content of 83.93 mg / g; after cation exchange resin catalyzed hydrolysis method of total sugar content was 404.91 mg / g, reducing sugar content was 368.46 mg / g. Percentage of total reducing sugars before hydrolysis was 26.9% sugar, reducing sugar percentage reached 91.0% of total sugars after hydrolysis. Select high active dry yeast for fermenting yeast soy molasses powder. By single factor and orthogonal experiments to determine the optimal fermentation conditions: initial pH 5.0, fermentation temperature 29.0 ℃, yeast inoculum 6 g / L, the fermentation time was 44 h. Optimum fermentation conditions were applied on soybean molasses raw materials, raw materials adding an acidic protease molasses powder hydrolyzate and soy molasses added to acidic protease hydrolyzate fermentation four cases.
After completion of the fermentation by gas chromatography analysis of the drinker. Test to get a drinker as follows: direct fermentation of raw drinker was 30.29 mg / g, the amount of alcohol fermentation enzyme raw materials for 61.82 mg / g, the amount of alcohol after fermentation feedstock hydrolysis was 157.99 mg / g, hydrolytic enzyme fermentation feedstock for the drinker 121.71 mg / g. Molasses powder in the application of the best combination of diet. After fermentation broth residual reducing sugar content of 10.77 mg / g, the residual total sugar content of 21.83 mg / g, the appearance of the sugar content of 6 oBx, pH 4.37, the wine was 15.8%.
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Sulfuric acid + activated carbon + law + NaHSO_3 France potassium ferrocyanide, cane molasses after treatment with this method is used to dilute fermentation, lactic acid production at its L- by untreated 52.34g / L increased to 61.88g / L, production increased by about 18%. reducing molasses powder content of 83.93 mg / g; after cation exchange resin catalyzed hydrolysis method of total sugar content was 404.91 mg / g, reducing sugar content was 368.46 mg / g. Percentage of total reducing sugars before hydrolysis was 26.9% sugar, reducing sugar percentage reached 91.0% of total sugars after hydrolysis. Select high active dry yeast for fermenting yeast soy molasses powder. By single factor and orthogonal experiments to determine the optimal fermentation conditions: initial pH 5.0, fermentation temperature 29.0 ℃, yeast inoculum 6 g / L, the fermentation time was 44 h. Optimum fermentation conditions were applied on soybean molasses raw materials, raw materials adding an acidic protease molasses powder hydrolyzate and soy molasses added to acidic protease hydrolyzate fermentation four cases.
After completion of the fermentation by gas chromatography analysis of the drinker. Test to get a drinker as follows: direct fermentation of raw drinker was 30.29 mg / g, the amount of alcohol fermentation enzyme raw materials for 61.82 mg / g, the amount of alcohol after fermentation feedstock hydrolysis was 157.99 mg / g, hydrolytic enzyme fermentation feedstock for the drinker 121.71 mg / g. Molasses powder in the application of the best combination of diet. After fermentation broth residual reducing sugar content of 10.77 mg / g, the residual total sugar content of 21.83 mg / g, the appearance of the sugar content of 6 oBx, pH 4.37, the wine was 15.8%.
http://www.xyd-chem.com/35-molasses-powder-en.html
http://www.whxydchem.com/products_detai_en/id/20.html